Abstract

UDK: 547.56:582.728.4
 Phenolic compounds content and antioxidant capacity from Viscum album ssp. album Beck. leaves and twigs extracts were determined. Common mistletoe was collected from four hosts (Crataegus monogyna, Malus domestica, Prunus cerasifera, and Populus x canadensis.). Folin-Ciocalteu method, AlCl3 method, method with Arnow reagent, and acid-butanol assay were applied for determination of total phenols, flavonoids, phenolic acids and proanthocyanidins respectively. DPPH, ABTS and FRAP methods were applied in the determination of antioxidant capacity of the extracts. Total phenols were in the range 7.16-11.70 mg GAE/g DW, flavonoids 2.115-4.668 mg RE/g DW, phenolic acids 0.811-1.789 mg caffeic CAE/g DW, and proanthocyanidins 0.71-2.62 mg LCE/g DW. Content of phenols and flavonoids was higher in the leaves than the twigs. The highest antioxidant capacity in leaves (50.86-65.93 µmol Trolox equivalents/g DW) was determined for mistletoe collected from Crataegus monogyna while the highest capacity in twigs had mistletoe collected from Malus domestica (67.28-81.72 µmol Trolox equivalents/g DW). Also, high correlation has been noticed between total phenols, flavonoids, phenolic acids and antioxidant capacity for V. album leaves. For twigs, high correlation was obtained between phenols and antioxidant capacity, and in some moderate extends for proanthocyanidins.

Highlights

  • UvodEuropean mistletoe or Common mistletoe (Viscum album L.) belongs to Loranthaceae family

  • The plant is represented by three subspecies which can be found on approximately 46 different trees in Bosnia: ssp. album Beck., ssp. abietis (Wiesb.) Abromeit. and ssp. austriacum (Wiesb.) Vollmann

  • Viscum album ssp. album is the most abundant and it has been identified on 42 leafy trees and shrubs (TREŠTIĆ, 2015)

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Summary

Introduction

European mistletoe or Common mistletoe (Viscum album L.) belongs to Loranthaceae family. It is an evergreen semi-parasitic plant which grows on various host trees and shrubs. Album is the most abundant and it has been identified on 42 leafy trees and shrubs (TREŠTIĆ, 2015). It has been reported that V. album shows various pharmacological effects: Phenolic content and antioxidant capacity of Viscum album leaves and twigs from various host trees antioxidant and antidiabetic (GRAY and FLATT, 1999, ORHAN et al, 2005), vasodilator and antihypertensive properties (TENORIO-LOPEZ et al, 2005; OFEM, 2007), antiepileptic, antipsychotic and sedative (GUPTA et al, 2012). V. album shows immunostimulant, antimutagenic and anticancer effects (YESILADA et al, 1998; SIEGLE et al, 2001; SABOVA et al, 2010; HONG and LYU, 2012)

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