Phenolic compounds in strawberry (Fragaria x ananassa Duch.) microshoots

Olga Kotsupiy,Elena Ambros,Evgeniya Karpova,E.V Banaev,M.A Tomoshevich,Y.G Zaytseva

doi:10.1051/bioconf/20202400041

Abstract

The HPLC method was used to study the composition and contents of phenolic compounds in the microshoots of Fragaria × ananassa Duch. plantlets during the multiplication and in vitro rooting stages in comparison with field-grown plants. Phenolic compounds of the microshoots are predominantly represented by ellagic acid and its derivatives, as well as hydroxycinnamic and hydroxybenzoic acids, in contrast to the leaves of field-grown plants with a high level of flavonoids. The increased contents of individual hydroxybenzoic and hydroxycinnamic acids in the microshoots compared with the leaves of field-grown strawberry plants indicates a possible role of phenolic acids in plant adaptation under in vitro conditions.

Highlights

Cultivated strawberry (Fragaria × ananassa Duch.) is one of the most popular berry fruits in many countries of the world
The adaptation is achieved by quercetin derivatives and phenolic acids, during in vitro proliferation, it realized by hydroxybenzoic acids and ellagic acid derivatives, and during in vitro rooting, it is implemented with hydroxycinnamic acids
The results revealed higher contents of most phenolic compounds and elevated total phenolic contents in fieldgrown plants compared to in vitro plantlets

Summary

Introduction

Cultivated strawberry (Fragaria × ananassa Duch.) is one of the most popular berry fruits in many countries of the world. In vitro conditions are stressful for plants (high relative humidity, low gas exchange with the external environment, the presence of exogenous carbohydrates and growth regulators, and low CO2 absorption rate). These in vitro factors interfere the successful acclimatization of plants under non-sterile conditions. In this regard, it is of interest to expand the spectrum of biochemical indicators of the adaptive status of strawberry plants with secondary metabolites, including phenolic compounds, which can often serve as indicators of stress and adaptation [3]. A comparison of phenolic compositions in F. × ananassa plants cultivated at the different conditions will help identify the major compounds for the adaptations to each type of environments, including in vitro conditions

Methods

Results

Discussion

Conclusion