Phenolic Compounds from Polygonum chinense Induce Growth Inhibition and Apoptosis of Cervical Cancer SiHa Cells

Wei Chen,Yunfeng Zheng,Qin Yuan,Rong Chen,Li Ma,Xianmin Shen,Guoping Peng,Cunyu Li,Ji-Fu Wei

doi:10.1155/2020/8868508

Abstract

Cervical cancer is considered to be one of the most serious malignant tumors in women. Natural compounds have been considered as important sources in the search for new anticancer agents. Polygonum chinense (PC) has been used as herbal medicine and Chinese cool tea. By activity-guided of the extracts from PC, PCwater shows good growth inhibition on SiHa cell, then by chromatographic analysis (HPLC and HPLC-MS/MS), we found twelve components, seven were phenolic compounds (PHE), two PHE named ellagic acid and corilagin were found to show strong growth inhibition effects in SiHa cell dose-dependently, while the seven phenolic compounds showed low inhibition on the common human HcerEpic cell. Further research found ellagic acid and corilagin induced G2 phase cell cycle arrest by upregulating levels of P53, Bcl-2, caspase 3, and caspase 9, while the Bax was reduced. These results suggested that PHE from PC might have potential anticancer effects against SiHa cells by acting through the apoptosis pathway, PHE from PC might have the potential to be used as a nutraceutical for the prevention and treatment of ovarian cancer.

Highlights

Cervical cancer is the second most common female cancer worldwide [1]
After the analysis of the water extraction, we further found water extraction of Polygonum chinense (PC) was mainly composed of phenolic acids, in which 12 compounds were identified
This study found that the apoptosis of SiHa in cervical epithelial tumor cells induced by two polyhydroxyphenolic acids in the mother carbon was realized through this pathway

Summary

Introduction

Cervical cancer is the second most common female cancer worldwide [1]. There are more than half a million new cases and more than 200,000 deaths each year [2, 3]; in recent years, the incidence of cervical cancer in China is on the rise, and the incidence of cervical cancer tends to be younger [4, 5]. Many researchers have been trying to find more effective chemotherapy drugs to treat cervical cancer cells. Agents that suppress the proliferation of malignant cells by enhancing apoptosis may represent a useful mechanistic approach to both cancer chemoprevention and chemotherapy [8, 9]. We should develop more safe and effective therapeutic agents for cancer treatment [10]

Methods

Results

Discussion

Conclusion