Abstract
Hypochaeris laevigata var. hipponensis (Asteraceae) is an endemic plant from Algeria. In the current study, we analyzed for the first time its chemical composition, especially phenolic constituents of dichloromethane (DCM), ethyl acetate (EA), and n-butanol (BuOH) fractionsof the aerial parts of Hypochaeris laevigata var. hipponensis by liquid chromatography-mass spectrometry (LC-MS/MS). The number of phenolic compounds detected in DCM, EA, and BuOH fractions were found to be 9, 20, and 15, respectively. More specifically, 12 phenolic acids were detected. Among them, quinic acid, chlorogenic acid, and caffeic acid were the most abundant ones. Meanwhile, only seven flavonoids were detected. Among them, rutin, apigetrin, and isoquercitrin were the major ones. We also determined the total phenolic and flavonoid contents, and fraction EA showed the highest values, followed by BuOH, and DCM fractions. Furthermore, the antioxidant action was dictated by five methods and the tested plant fractions demonstrated a noteworthy antioxidant action.
Highlights
A large number of medicinal and aromatic plants grow spontaneously in the Edough Peninsula, such as plants of family Asteraceae which are rich in phenolic compounds, volatile oils, and other bioactive compounds
Results of the Cupric Reducing Antioxidant Capacity (CUPRAC) test of ethyl acetate (EA) and BuOH extracts exhibited a higher activity (A0.50 value: 1.48 ± 0.33 and 3.00 ± 0.98 μg/mL, respectively) than those of standards (BHT with 3.80 ± 0.00 and α-tocopherol with 10.20 ± 0.01 μg/mL).The results we found are similar to those of Gorinsteinet al. [31], who say that the highest capacities of polyphenolic compounds are measured with CUPRAC, and they are similar to the results of Prior et al [32], who found that the CUPRAC method showed the highest antioxidant activities compared with other antioxidants tests
The LC-MS/MS lead a total of 23 chemical compounds in the three extracts
Summary
A large number of medicinal and aromatic plants grow spontaneously in the Edough Peninsula, such as plants of family Asteraceae which are rich in phenolic compounds, volatile oils, and other bioactive compounds. It is fundamental to extend the knowledge of the chemical composition of some plants of this family [1]. According to Stebbins [2], Hypochaeris is a smallgenusof the Asteraceae family, which contains about 50 species. Hypochaeris radicata is medically important and has anti-inflammatory, anticancer, antioxidant [5], antibacterial [6], antifungal [7] properties, and antidiuretics. It is used for the treatment of jaundice, rheumatism, dyspepsia, constipation, hypoglycemia, and kidney problems in the traditional medicinal practice of Tamil Nadu, India [8]. No scientific validation has been made for this species for medicinal purposes
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