Abstract
Chemical investigation of methanol extract of Ocimum basilicum L. resulted the isolation and identification of twelve phenolic compounds as p-hydroxy benzoic acid, ferulic acid, gallic acid, p-qumaric acid, benzoic acid, kaempferol, catechin, quercetin, chlorogenic acid, caffeic acid, cinnamic acid and ellagic acid. The structures of these compounds were confirmed by Chromatographic, UV, MS, 1H-NMR and 13C-NMR spectral data. The cytotoxic activity of the methanol extract of Ocimum basilicum showed strong cytotoxic activity against colon (HCT116) and liver (HEPG2) carcinoma cell line, where IC50 of the two human cell line were 27 μg/ml and 34.5 μg/ml, respectively.
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