Abstract
The preventive effects of phenolic compounds on insulin signalling and on both glucose production and uptake were studied in insulin-responsive human HepG2 cells treated with high glucose. The influence of insulin in different-dose insulin and at different action times on the IR of HepG2 cells, and changes of glucose uptake and glycogen level were detected by using cell culture glucose uptake analysis. Seven compounds, agrimonolide (1), desmethylagrimonolide (2), quercetin (3), luteolin (4), luteolin-7-O-glucoside (5), kaempferol (6), and apigenin (7), were used. The IR model was successfully established in HepG2 cells after the action of 5 × 10−7 M insulin for 24 h. The analysis of glucose uptake showed that 1–3 samples had significant glucose lowering activity and exhibited no difference with metformin (P > 0.05). All compounds had low cytotoxicity in HepG2 cells within the test concentration. Compounds 1 and 2 pre-treatment also prevented the inactivation of the AKT pathway and AMPK, which play an important role in glycometabolism.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
