Abstract

Nowadays traditional medicine, a source of several bioactive molecules for therapeutic purposes, has become a cure for various diseases. In fact, the evaluation of plant exploitation has become progressively significant and this for their therapeutic effects of many traditional medicines may be due to the immense presence of natural antioxidants. In this respect, Retama reatam was chosen among six herbal Tunisian plants traditionally known for their therapeutic virtues, as the best source of total polyphenol content (1122,927g GEA/g extract) with an important extraction yield as ethanol is the solvent. The Soxhlet extraction always has the lowest value in total polyphenol content (164,857 GEA/g extract) for such solvent. The total flavonoid content of the different extracts is not significantly different from each other. However, the yield extraction remains higher for ethanolic extraction exceeding a value of 26.7%. Concerning antioxidant activity of Retama reatam, results suggest that supercritical CO2 extraction can be used as an efficient alternative for pre-treatment to eliminate fatty compounds and therefore evaluating oxygen radical absorbance capacity values.

Highlights

  • Herbal materials are known as a prominent medicinal source of polyphenols flavonoids and represent a class of bioactive molecules that are closely related to multiple health benefits

  • Total flavonoid content (TFC) is characterized by a modification of the Yang procedure proposed by Pekal et al (Pękal and Pyrzynska 2014)

  • Comparing the total polyphenol content value by maceration technique lasted after 6 days, 1123-mgGEA/g dry extract, Total phenolic content (TPC) for other extracts following different extractions is lower, this may be due to the extraction time at a given temperature and influenced by ethanol concentration [12]

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Summary

Introduction

Herbal materials are known as a prominent medicinal source of polyphenols flavonoids and represent a class of bioactive molecules that are closely related to multiple health benefits. The interest in plant and herbal materials has become gradually significant may be due to the presence of natural antioxidants such as phenolic plant compounds and flavonoids. In this respect, the development of a standard method for efficient and rapid extraction of these secondary metabolites remains a challenge. Very few books have been devoted to Retama reatam which is spontaneous shrub belonging to the Fabaceae family, locally known as R'tem This virtue plays an important ecological role and is widely used in dune stabilization and soil fixation [1]. It is usefully used for the treatment of several diseases such as diabetes, Conforti et al [2], eczema and hypertension, Alfalluos et al [3]

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