Phenogenomics of the gingival fibroblast interaction with implant materials- a standardized system to determine material/surface potential for connective tissue attachment

Abstract

Event Abstract Back to Event Phenogenomics of the gingival fibroblast interaction with implant materials- a standardized system to determine material/surface potential for connective tissue attachment Gethin Owen1, Ping Huang1, Sadaf G. Kashani1, Lari Hakkinen1 and Hannu Larjava1 1 University of British Columbia, Oral, Biol & Med Sci, Canada The health of the soft-tissue surrounding a dental implant is an integral part of successful dental implant treatment. Human and animal models have shown that the peri-implant connective tissue contains relatively low numbers of fibroblasts, little or no blood vessels, similar to scar-like tissue. Such a tissue forms a poorly functional attachment to the implant surface unlike the gingival attachment to teeth. We report on an in vitro test at the phenotype and genotype level to quickly assess the scar forming potential of implant materials/surfaces. To test our methodology 3 titanium surfaces were used, namely pickled (PT) (Sa =0.3 µm), sandblasted (S) and sand blasted acid etched (SLA) (both Sa =1.2 µm). The surfaces were characterized by scanning electron microscopy (SEM). The initial gingival fibroblast (GF) response was quantified comparing cell adhesion, cell proliferation, immunolabelling and area of cell-implant contact with a focused ion beam/scanning electron microscope (FIB/SEM). Extracellular matrix protein (ECM) synthesis, angiogenesis potential and gene expression on each material surface were also quantified at 1 and 7 days. GFs cultured on all surfaces had distinct morphology after 1 day. On PT they were well spread. On S some cells were well spread and others had a stellate shape. All cells on SLA were stellate. Mature focal adhesions (FA) were visible on PT and S. Stellate shaped GFs cultured on S and SLA had small FA on the edges of surface features. Cross-sections of cell/implant with FIB/SEM confirmed this finding. FA area of adhesion was greatest on SLA which resulted in stronger cell adhesion as assessed by a cell detachment assay. After 1 day cell proliferation on SLA was significantly lower than PT and S but there was no difference after 7 days. Significant differences in expression of wound healing genes were seen relative to tissue culture plastic. Increased roughness caused a decrease in collagen I, III and decorin genes whereas matrix metalloproteinase (MMP)-1 increased after 1d. MMP-3 expression increased only in SLA after 7d. VEGF was highly expressed in SLA but the tube formation assay with HUVEC cells showed no angiogenic effect. ECM synthesis quantification showed significant differences in collagen I, decorin and fibronectin-EDA production. Production was highest on PT and the ECM had orientated with the topography in PT and S but not with SLA. This phenogenomic test suggests that surface topography is effective in modifying wound healing behavior at the GF phenotype, proteome and gene level. The investigation was funded by ITI Research Grant No.964-2013 Keywords: Implant, Cell response, tissue compatibility, surface topolography Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: General Session Oral Topic: Biomaterials in dental applications Citation: Owen G, Huang P, Kashani SG, Hakkinen L and Larjava H (2016). Phenogenomics of the gingival fibroblast interaction with implant materials- a standardized system to determine material/surface potential for connective tissue attachment. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02438 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Gethin Owen Ping Huang Sadaf G Kashani Lari Hakkinen Hannu Larjava Google Gethin Owen Ping Huang Sadaf G Kashani Lari Hakkinen Hannu Larjava Google Scholar Gethin Owen Ping Huang Sadaf G Kashani Lari Hakkinen Hannu Larjava PubMed Gethin Owen Ping Huang Sadaf G Kashani Lari Hakkinen Hannu Larjava Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.