Phenazine-1-carboxylic acid-producing Pseudomonas synxantha LBUM223 alters the transcriptome of Streptomyces scabies, the causal agent of potato common scab

Abstract

Common scab of potato is an important disease caused by Streptomyces scabies. Biological control of this disease was previously demonstrated under controlled and field conditions using Pseudomonas synxantha LBUM223, a phenazine-1-carboxylic acid (PCA)-producer. To better understand the impact of PCA on the pathogen, RNA-seq analyses of S. scabies confronted with wild-type LBUM223, its isogenic PCA-deficient mutant and purified PCA were performed. Following exposure to wild-type LBUM223 or purified PCA (but not to the isogenic PCR-deficient mutant), 12%–14% of all S. scabies genes were differentially expressed, including key genes involved in pathogenicity/virulence, mycelium differentiation, and increased oxidative stress in S. scabies.