Phellinus noxius: molecular diversity among isolates from Taiwan and its phylogenetic relationship with other species of Phellinus based on sequences of the ITS region

Jyh-Nong Tsai,Wen-Hsiung Ko,Pao-Jen Ann,Wen-Hsui Hsieh,Ruey-Fen Liou

doi:10.1186/s40529-017-0162-1

Abstract

BackgroundAnalysis of phylogenetic relationship of 91 isolates of Phellinus noxius obtained from 46 plant species in Taiwan did not show distinct grouping based on ITS sequences.Results However, the ITS nucleotides showed 20 different kinds of variations including single nucleotide polymorphisms, deletion and insertion in ITS1 and ITS2, but none in 5.8 S. The Taiwanese isolates of P. noxius were dividable into long (type L), median (type M) and short (type S) groups based on ITS sequence length. Two isolates with identical ITS sequence belonged to types L. Type M with 72 isolates was further divided into 33 subtypes, while types S with 17 isolates was further divided into two subtypes.ConclusionPhylogenetic analysis of ITS sequences among Phellinus species showed that isolates of P. noxius were in the same clade distinctly separated from other Phellinus species.

Highlights

Analysis of phylogenetic relationship of 91 isolates of Phellinus noxius obtained from 46 plant species in Taiwan did not show distinct grouping based on ITS sequences
Results from this study showed that the isolates of P. noxius from Taiwan can be divided into type L, type M and type S based on ITS sequence length
Phylogenetic analysis of ITS sequences among Phellinus species showed that isolates of P. noxius were in the same clade distinctly separated from other Phellinus species (Fig. 2)

Summary

Introduction

Analysis of phylogenetic relationship of 91 isolates of Phellinus noxius obtained from 46 plant species in Taiwan did not show distinct grouping based on ITS sequences. Brown root rot caused by Phellinus noxius (Corner) G. Is widespread among tropical countries in Southeast Asia, Africa, Oceania, Central America and the Caribbean (Pegler and Waterston 1968). In China, it has been reported from the tropical Hainan Island (Tai 1979). In Japan, it was found on the subtropical island of Okinawa (Abe et al 1995). The pathogen attacks more than 120 species of fruit and ornamental trees in both topical and subtropical districts in Taiwan (Ann et al 1999; Chang and Yang 1998). Among the approximately 200 plant species listed as hosts of P. noxius in the world, about half

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Discussion

Conclusion