桑黃（Phellinus linteus）於攪拌式生物反應器中菌絲體生質量及生理活性物質之生產

Abstract

The influences of cultivation conditions on mycelial biomass and bioactive compounds produced by a medicinal mushroom Phellinlls Iinleus in a 5-L stirred-tank bioreactor were studied. The results showed that the optimal inoculum ratio, aeration rate and agitation rate for the production of mycelia biomass were 5%, 1.5 vvm and 125rpm, respectively. Then, the mushroom was grown under the optimal cultivation conditions (inoculum ratio 5%, aeration rate 1.5vvm and agitation rate 125 rpm) in a 5-L stirred-tank bioreactor after 20 days of incubation time, and the maximal production of mycelial biomass, mycelial biomass formation rate and mycelial biomass yield were 8.86g/L, 0.44g/L/day and 0.44g/g glucose, respectively. These results were 1.93-, 1.91- and 1.91-fold of the maximal production of mycelial biomass, mycelial biomass formation rate and mycelial biomass yield of P Iinleus under the basal cultivation conditions (inoculums ratio 10%, aeration rate 1.0vvm and agitation rate 100 rpm). During 20 days of incubation time, the total phenolic content and values of Trolox equivalent antioxidative capacity for P. Iinlcns culture broth were increased with production of mycelia biomass. However, the content of s-1,3-glucan was decreased during incubation time.