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Abstract
Insulin-containing microcapsules were prepared by a layer-by-layer (LbL) deposition of poly(allylamine hydrochloride) (PAH) and polyanions, such as poly(styrenesulfonate) (PSS), poly(vinyl sulfate) (PVS), and dextran sulfate (DS) on insulin-containing calcium carbonate (CaCO3) microparticles. The CaCO3 core was dissolved in diluted HCl solution to obtain insulin-containing hollow microcapsules. The microcapsules were characterized by scanning electron microscope (SEM) and atomic force microscope (AFM) images and ζ-potential. The release of insulin from the microcapsules was faster at pH 9.0 and 7.4 than in acidic solutions due to the different charge density of PAH. In addition, insulin release was suppressed when the microcapsules were constructed using PAH with a lower molecular weight, probably owing to a thicker shell of the microcapsules. The results suggested a potential use of the insulin-containing microcapsules for developing insulin delivery systems.
Highlights
Polyelectrolyte layer-by-layer (LbL) films can be prepared by an alternate and repeated deposition of polymeric materials on a solid surface through electrostatic interactions [1,2], hydrogen binding [3,4], host-guest complexation [5,6], and biological affinity [7]
Insulin-containing microcapsules were prepared by depositing LbL films on the surface of insulin-loaded
Insulin is encapsulated in the cavity in the form of insulin-PSS aggregates created through electrostatic interactions because the microcapsules were prepared by using CaCO3 particles containing PSS and insulin
Summary
Polyelectrolyte layer-by-layer (LbL) films can be prepared by an alternate and repeated deposition of polymeric materials on a solid surface through electrostatic interactions [1,2], hydrogen binding [3,4], host-guest complexation [5,6], and biological affinity [7]. Hollow microcapsules have been constructed by coating the surface of microspheres with LbL films followed by dissolution of core material [20–24]. Microcapsules containing unstable compounds, such as proteins and genes, can be successfully prepared [25–27] In this context, we reported that insulin can be built into LbL films by an alternate deposition of polymers and insulin to enable pH-controlled release of insulin [28–30]. Insulin was released from the LbL films and microspheres at neutral pH while the release was suppressed at acidic pH, suggesting a possible use of them for developing oral administrations of insulin. The LbL microcapsules were prepared by coating insulin-containing CaCO3 particles with LbL films, followed by dissolution of CaCO3 core in acidic solution
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