Phase-Shifted Direction Adaptation of the Vestibulo-Ocular Reflex in Cat

Abstract

The ability of the vestibulo-ocular reflex (VOR) to alter the phase of the motor output relative to the sensory input is examined. Alert cats were trained for 2 h with 0.25 Hz sinusoidal horizontal vestibular and vertical optokinetic rotational stimuli. In each experiment the optokinetic training stimulus was phase shifted by 0 degree, +45 degrees, -45 degrees, or 90 degrees from the vestibular stimulus. Vertical and horizontal eye movements were measured during horizontal rotations in darkness before and after the training procedure. Phase-advance experiments (+45 degrees) produced an adaptive vertical VOR with a mean phase of +28 degrees. After phase-delay experiments (-45 degrees), the adapted VOR had a mean phase of -19 degrees. The peak adaptive change in VOR gain was at or near the 0.25 Hz training frequency in each experimental group, but the gain depended in a complex manner on the testing frequency and the degree of phase shift of the training stimulus. Training with a 45 degrees phase-delayed optokinetic stimulus produced an adaptive vertical VOR with a gain that was relatively higher at frequencies below the training stimulus than at those that were above. Training with a 45 degrees phase-advanced optokinetic stimulus produced an adaptive vertical VOR with a gain that was higher at frequencies above the training frequency than at those that were below. During training with a phase-shifted optokinetic stimulus, adjustment of the relative efficacies of two neural pathways, a velocity pathway and an integrating pathway, could account for gain dependence on testing frequency and phase shift. This was corroborated by a model of the VOR that incorporates parallel velocity and integrating pathways. Data from 45 degrees phase advances were fit by increasing the gain of the velocity versus integrating pathway, whereas 45 degrees phase delay data were fit by decreasing the gain of the direct versus integrating pathway. The models altered the time constants of either the common oculomotor integrator or the velocity storage mechanism.