Phase locking of neuronal responses to the vertical refresh of computer display monitors in cat lateral geniculate nucleus and striate cortex

Abstract

The proliferation of low-cost microcomputer systems has led to the use of these systems as alternatives to expensive display devices for visual physiology and psychophysics experiments. The video displays of these systems often lack the flexibility of achieving wide linear luminance ranges and high vertical refresh rates — two parameters which may influence data acquisition. We have examined the responses of neurons and pairs of neurons in cat LGN and striate cortex to bar and sinusoidal grating stimuli generated by a conventional PC-based VGA graphics card and displayed on a NEC Multisync + color monitor with a 60 Hz vertical (display) refresh rate. Responses to these stimuli were autocorrelated and power spectral densities (PSD) were calculated, revealing that the majority of simple and complex cortical cells and nearly all LGN cells exhibited significant peaks in their autocorrelations at 16.7 ms and in the PSD at 60 Hz. Responses to identical stimuli generated with an optical bench using an incandescent light source contained no power at 60 Hz. Furthermore, cross-correlations between the spike trains of neuron-pairs were severely contaminated by peaks directly attributable to the entrainment of the two elements of the pair to the vertical refresh signal. Thus, we suggest that the use of conventional computer displays introduces a temporal artifact into neuronal spike trains in both single and multiple spike train analysis.