Phase holograms for the three-dimensional patterning of microparticles in a traveling wave field

Abstract

Acoustic fields can generate radiation forces that can align particles in patterns. Forces from standing waves pattern particles in three dimensions (3-D) at either nodal or anti-nodal regions. These patterns can be utilized to form 3-D microstructures for applications in tissue engineering or fabrication of layered materials. However, standing waves require more than one transducer or a reflector, which can complicate implementation. Here, we developed a method to suspend and align microspheres using a traveling wave from a single transducer. Acoustic fields were shaped to align polyethylene microspheres mimicking tissue cells in parallel planes along the axis of the transducer. A Bessel-like beam was developed using diffraction theory and an iterative angular spectrum approach were used to design phase holograms to shape pressure fields. Gor’kov potential was used to calculate radiation forces while minimizing the axial forces to create a stable trap. The resulting pressure fields and particle patterns matched predictions with a similarity index >0.92, where 1 is a perfect match. The transverse radiation force was ten times each microsphere’s weight and comparable to the standing wave radiation forces. Next steps are in vivo implementation of cell patterning for tissue engineering. [Work supported by NIH K25-DK132416 and P01-DK043881.]