Abstract
Two-dimensional mass spectrometry (2D MS) is a tandem mass spectrometry method that relies on manipulating ion motions to correlate precursor and fragment ion signals. 2D mass spectra are obtained by performing a Fourier transform in both the precursor ion mass-to-charge ratio (m/z) dimension and the fragment ion m/z dimension. The phase of the ion signals evolves linearly in the precursor m/z dimension and quadratically in the fragment m/z dimension. This study demonstrates that phase-corrected absorption mode 2D mass spectrometry improves signal-to-noise ratios by a factor of 2 and resolving power by a factor of 2 in each dimension compared to magnitude mode. Furthermore, phase correction leads to an easier differentiation between ion signals and artefacts, and therefore easier data interpretation.
Highlights
Molecules 2021, 26, 3388. https://Two-dimensional mass spectrometry (2D MS) is a tandem mass spectrometry method that relies on manipulating ion motions to correlate precursor and fragment ion signals [1,2,3]
The pulse sequence and data processing have been optimized for analytical use [12,13,14,15]. 2D FT-ICR MS has been used for applications ranging from small molecules and agrochemicals, to polymers, bottom-up and top-down proteomics [16,17,18,19,20,21,22,23,24]. 2D MS has been adapted for high-resolution analysis of precursor ions over narrower mass-to-charge ratio (m/z) ranges [25]
We have for the first time obtained a phase-corrected absorption mode
Summary
Two-dimensional mass spectrometry (2D MS) is a tandem mass spectrometry method that relies on manipulating ion motions to correlate precursor and fragment ion signals [1,2,3]. Various 2D MS methods have been developed for quadrupolar ion traps, linear ion traps, and Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS) [4,5,6,7,8]. In addition to precursor-fragment correlations, two-dimensional partial correlation mass spectrometry (2D PC MS) has been developed to explore correlations between complementary fragment ion signals [9]. Since 2D MS relies on Fourier transformation in both dimensions, one area in which its performance can be improved is the phase correction for absorption mode mass spectrometry.
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