Abstract
We present propagation-based phase-contrast tomography of mouse sciatic nerves stained with osmium, leading to an enhanced contrast in the myelin sheath around the axons, in order to visualize the threedimensional (3D) structure of the nerve. We compare different experimental parameters and show that contrast and resolution are high enough to identify single axons in the nerve, including characteristic functional structures such as Schmidt-Lanterman incisures.
Highlights
0.2 -/1e-05/0.01 sample and the detector as well as the choice of the regularization parameters α1 or δ β and α2 which are defined as in [4]
We present propagation-based phase-contrast tomography of mouse sciatic nerves stained with osmium, leading to an enhanced contrast in the myelin sheath around the axons, in order to visualize the threedimensional (3D) structure of the nerve
A standard tool for imaging of nerve tissue is electron microscopy which leads to high resolution reconstructions of the inner structure of the nerve but is limited to small volumes. (Hard) x-ray computed tomography is a promising approach for 3D imaging of relatively large volumes, especially by using the phase shift instead of the absorption of the beam, as this leads to superior contrast in soft tissue
Summary
0.2 -/1e-05/0.01 sample and the detector as well as the choice of the regularization parameters α1 or δ β and α2 which are defined as in [4]. We present propagation-based phase-contrast tomography of mouse sciatic nerves stained with osmium, leading to an enhanced contrast in the myelin sheath around the axons, in order to visualize the threedimensional (3D) structure of the nerve.
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