Abstract
Singlet oxygen and superoxide production by rose bengal photoactivation leads to rapid electrophysiological changes and arrhythimias. To investigate which intermediate is causeative and to probe possible mechanisms, hearts ( n = at least 6/group) were perfused aerobically for 10 min without rose bengal followed by 5 min with rose bengal before illumination for 20 min. In controls, all or most hearts exhibited ventricular premature beats, ventricular tachycardia, and complet atrioventricular block. Most antioxidants tested had no protective effect; histidine, however, significantly delayed the onset of elctrocardiographic (ECG) changes. In further studies, two antiarrhythmic agents (quinidine and verapamil) had no or little protective effect, whereas R56865 significantly delayed the onset of ECG changes and reduced the incidence of arrhytmias. However, spectrophotometric and laser pulse radiolysis studies showed that this apparent protective effect might have resulted from an interaction between R56865 and the rose bengal molecule, leading to a reduction in singlet oxygen production. In conclusion, the electrophysiological changes induced by rose bengal photoactivation are likely to be due to singlet oxygen; antiarrhythmic drugs appear to be unable to protect against the injury unless there is some interaction with the photoactivation process.
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