Abstract
BackgroundMedicinal plants have proven their importance as a valuable source of molecules with therapeutic potential. Monotheca buxifolia (Falc.) A. DC. (family: Sapotaceae) is traditionally used as a hematinic, laxative, digestive, anthelmintic, antipyretic, and in the treatment of gastro-urinary disorders. To provide scientific evidence for its folkloric use, the present study investigated Monotheca buxifolia fruit hydro-ethanolic extract (MBHE) for its prospective antinociceptive, anti-inflammatory and antipyretic activities. MBHE was eluted through column chromatography to isolate the bioactive secondary metabolites which may probably involve in its beneficial properties.MethodsThe phytochemical constituents in MBHE was elucidated using UV, IR, 1H-NMR, 13C NMR, 2D-NMR spectra in combination with EIMS and FAB-MS spectrometric techniques and comparison with literature data of related compounds. The antinociceptive activity of MBHE was evaluated in the acetic acid induced abdominal constriction assay; the anti-inflammatory potential was assessed in the carrageenan induced paw edema, while the antipyretic effect was tested against brewer’s yeast induced pyrexia in BALB/c mice at doses of 50, 100 and 150 mg/kg.ResultsElution of MBHE along with various characterization techniques led to the isolation of oleanolic acid and isoquercetin. Significant attenuation of chemical induced nociception was observed with MBHE at tested doses of 50 mg/kg (P < 0.01, 68.87 %), 100 mg/kg (P < 0.01, 68.87 %) and 150 mg/kg (P < 0.001, 83.02 %). During a duration of 1–5 h in the carrageenan induced paw edema assay, significant ameliorative effect (P < 0.01, P < 0.001) was demonstrated by MBHE at 50 mg/kg (22.94–20 %), 100 mg/kg (33.23–21.13 %) and 150 mg/kg (38.23–25 %). MBHE also significantly alleviated the brewer’s yeast induced pyrexic response when tested at doses of 50 mg/kg (P < 0.05 in 2nd h), 100 mg/kg (P < 0.05, P < 0.01 and P < 0.001 in 1–5 h) and 150 mg/kg (P < 0.01 and P < 0.001 in 1–5 h).ConclusionThese findings suggest that Monotheca buxifolia possess pain, inflammation and pyrexia ameliorating properties, probably mediated by the presence of oleanolic acid and isoquercetin contents, though the involvement of other important phytochemicals constituents cannot be ignored.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1257-z) contains supplementary material, which is available to authorized users.
Highlights
Medicinal plants have proven their importance as a valuable source of molecules with therapeutic potential
M. buxifolia fruit is used as hematinic, laxative, purgative, vermicidal, antipyretic, and for the management of gastro-urinary disorders [4,5,6,7]
Two new compounds, buxifoline-A as alkaloid and buxilide as pyrone were isolated from the ethylacetate fraction of M. buxifolia fruit [9]
Summary
Medicinal plants have proven their importance as a valuable source of molecules with therapeutic potential. To provide scientific evidence for its folkloric use, the present study investigated Monotheca buxifolia fruit hydro-ethanolic extract (MBHE) for its prospective antinociceptive, anti-inflammatory and antipyretic activities. Discovering excellent remedies for diseases that are efficacious, economical and having minimum adverse effects is the need of the hour For discovering such products, medicinal plants are considered as best choice, as they provide a wide range of bioactive compounds, making them a rich source of different types of medicines [1]. M. buxifolia is mainly used for fuel, fodder, small timber, roof thatching materials, and notably used as fence around cultivated fields due to its thorny nature This species yields fruits, locally called Gurguri, which provides a source of income for the local inhabitants [3]. The family Sapotaceae is widely studied for antimicrobial [10, 11], antioxidant [8], antipyretic [12], CNS depressant [13], anti-inflammatory [14, 15], anthelmintic [16] and antinociceptive activities [12, 17] in various in vitro and in vivo experimental models
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