Abstract
BackgroundVarious extracts of Centella asiatica (Apiaceae) and its active constituent, asiaticoside, have been reported to possess wound healing property when assessed using various in vivo and in vitro models. In an attempt to develop a formulation with accelerated wound healing effect, the present study was performed to examine in vivo efficacy of asiaticoside-rich hydrogel formulation in rabbits.MethodsAsiaticoside-rich fraction was prepared from C. asiatica aerial part and then incorporated into polyvinyl alcohol/polyethylene glycol (PVA/PEG) hydrogel. The hydrogel was subjected to wound healing investigation using the in vivo incision model.ResultsThe results obtained demonstrated that: i) the hydrogel formulation did not cause any signs of irritation on the rabbits’ skin and; ii) enhanced wound healing 15% faster than the commercial cream and > 40% faster than the untreated wounds. The skin healing process was seen in all wounds marked by formation of a thick epithelial layer, keratin, and moderate formation of granulation tissues, fibroblasts and collagen with no fibrinoid necrosis detected.ConclusionThe asiaticoside-rich hydrogel developed using the freeze-thaw method was effective in accelerating wound healing in rabbits.
Highlights
It is currently estimated that approximately six million people globally suffer from chronic wounds
The wound healing potential of C. asiatica extracts, or ‘pegaga nyonya’ as it is known to the Malay, have been justified in both experimental and clinical evaluations [17,18,19,20,21], with most of these studies reporting that asiaticoside as the main active constituent producing the said effect
Acute dermal irritation test A skin irritation test of C. asiatica hydrogel formulation was performed on three rabbits
Summary
It is currently estimated that approximately six million people globally suffer from chronic wounds. Centella asiatica extract showed its activity in tissue regeneration [14], cell migration [15] and wound repair process by promoting fibroblast proliferation and collagen synthesis [16]. The wound healing potential of C. asiatica extracts, or ‘pegaga nyonya’ as it is known to the Malay, have been justified in both experimental and clinical evaluations [17,18,19,20,21], with most of these studies reporting that asiaticoside as the main active constituent producing the said effect. Various extracts of Centella asiatica (Apiaceae) and its active constituent, asiaticoside, have been reported to possess wound healing property when assessed using various in vivo and in vitro models. In an attempt to develop a formulation with accelerated wound healing effect, the present study was performed to examine in vivo efficacy of asiaticoside-rich hydrogel formulation in rabbits
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