Abstract
Salvia officinalis (Lamiaceae) has been used in south of Brazil as a diary homemade, in food condiment and tea-beverage used for the treatment of several disorders. The objective of this study was to characterize chemical compounds in the hydroalcoholic (ExtHS) and aqueous (ExtAS) extract from Salvia officinalis (L.) by gas chromatography-mass spectrometry (GC-MS) and by high-resolution electrospray ionization mass spectrometry (ESI-QTOF MS/MS), evaluate in vitro ability to scavenge the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH•) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS•+), catalase (CAT-like) and superoxide dismutase (SOD-like) activity, moreover cytotoxic by MTT assay, alterations on cell morphology by giemsa and apoptotic-induced mechanism for annexin V/propidium iodide. Chemical identification sage extracts revealed the presence of acids and phenolic compounds. In vitro antioxidant analysis for both extracts indicated promising activities. The cytotoxic assays using tumor (Hep-2, HeLa, A-549, HT-29 and A-375) and in non-tumor (HEK-293 and MRC-5), showed selectivity for tumor cell lines. Immunocytochemistry presenting a majority of tumor cells at late stages of the apoptotic process and necrosis. Given the results presented here, Brazilian Salvia officinalis (L.) used as condiment and tea, may protect the body against some disease, in particularly those where oxidative stress is involved, like neurodegenerative disorders, inflammation and cancer.
Highlights
The importance of plants traditionally used by the population can lead us to search for new active compounds with application in chemoprevention or anticancer therapy
The highest intense peaks shown in the ESIQTOF analysis from the hydroalcoholic extract (Table II, entries 1-4) were the deprotonated molecules [M-H]- at m/z 345.2086 and m/z 455.3773, which correspond to a nominal mass of 346 u and 456 u, respectively
Major constituents expressed as peak area percentage of hydroalcoholic extract of S. officinalis identified by gas chromatography-mass spectrometry (GC-MS) analysis
Summary
The importance of plants traditionally used by the population can lead us to search for new active compounds with application in chemoprevention or anticancer therapy. Tumor cells exposed to sage extracts may reduce cell viability by mechanisms not elucidated, but it is believed that this plant exerts its antitumor effect by changing the redox equilibrium, inducing apoptosis pathways and cell cycle arrest (Zare Shahneh et al 2013, Vasko et al 2014). Given sage extract promising biological activities, the aim of this study is to investigate the major compounds presented in the hydroalcoholic and aqueous sage extract, its antioxidant activity and cytotoxic effect against well-known tumor cell lines: human laryngeal carcinoma (Hep-2), human cervix adenocarcinoma (HeLa), human lung carcinoma (A549), human colorectal adenocarcinoma (HT-29) and human melanoma (A-375). This study evaluated the morphological alterations and induction of apoptosis and necrosis after exposure to hydroalcoholic and aqueous sage extract
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
