Pharmacological in‑vitro investigation for digestive property and in‑vivo anti‑ulcer activity of Pep‑Up Syrup

Abstract

Objective: To investigate in‑vitro digestive property and in‑vivo anti‑ulcer activity of Pep‑Up Syrup in aspirin‑induced acute gastric ulcer. Materials and Methods: The in‑vitro investigation for digestive property was performed by means of evaluating amylolytic, lipolytic and proteolytic activity in comparison with blank. For in‑vivo evaluation of Pep‑Up Syrup, aspirin‑induced acute gastric ulcer model in rats was used. Selected animals were divided in to three groups where each group was consisting of six animals. Group‑I, II and III was considered as normal control, disease control and Pep‑Up Syrup treated, respectively. Pep‑Up Syrup (3 mL/kg/day) treatment was provided for 7 days orally. Ulcer index, gastric wall mucus content, lipid peroxidation level in stomach tissue and tissue antioxidant parameters like superoxide dismutase (SOD), reduced glutathione and catalase enzyme activity were carried out. Histopathology of stomach tissue was also performed. Statistical Analysis: Statistical calculations were done by analysis of variance (ANOVA) followed by post‑hoc Bonferroni’s test and results were expressed as Mean ± SEM. Results and Discussion: Pep‑Up Syrup showed noticeable amylolytic, lipolytic and proteolytic activity. Pre‑treatment of Pep‑Up Syrup showed significant protection against ulceration and aspirin‑induced loss of gastric wall mucus content. Pre‑treatment of Pep‑Up Syrup also showed significant increase in tissue antioxidant parameters. Histopathology study revealed that Pep‑Up Syrup provides significant cyto‑protection against aspirin‑induced mucosal damage. Conclusions: On the basis of study data it can be concluded that Pep‑Up Syrup possesses property of digesting starch, lipids and proteins. Data also revealed that Pep‑Up Syrup has antiulcer activity against aspirin‑induced acute gastric ulcer. Key words: Antioxidant, in‑vitro digestive property, in‑vivo anti‑ulcer activity, Pep‑Up Syrup