Pharmacological evaluation of Thuja occidentalis for the attenuation of nephropathy in streptozotocin-induced diabetes rats

Abstract

In diabetic nephropathy (DN), chronic hyperglycemia causes oxidative stress and dyslipidemia, resulting in chronic renal damage. To mitigate the effects of hyperglycemia-induced kidney impairment, it may be a potential mechanism for future research This study aims to evaluate the nephroprotective efficacy of Thuja occidentalis in streptozotocin (STZ)-nicotinamide (NAD)-induced DN in male Wistar rats. Single dose of STZ (65 mg/kg, i.p.) was used to induce DN in Wistar rats. Serum insulin, glucose, renal function test, lipid profile, oxidative stress, inflammatory markers & histopathology were used to evaluate nephropathy. Wistar rats were treated with varying doses of hydroalcoholic extracts of Thuja occidentalis (HAETO) and Glimepiride for 30 days. Thuja occidentalis considerably correct the lipid profile levels and oxidative stress caused by hyperglycemia and the restoration of renal functions, indicating that it effectively treats DN. These findings indicate that the HAETO effectively inhibits the development of DN. The hypoglycemic, antioxidant, and anti-inflammatory properties of Thuja occidentalis are thought to be responsible for the nephroprotective benefit. • Accumulation of AGE's plays a significant role in establishing Diabetes Nephropathy. • Oxidative stress may be a promising strategy for reversing hyperglycemia-induced kidney impairment. • STZ is used in experimental animals to induce diabetes mellitus. • In diabetes, enhanced levels of TNF-α and interleukins cause microvascular permeability and renal function loss. • Thuja Occidentalis's antioxidant activity may play a crucial part in the recovery of kidney tissues.