Pharmacological correction of white rats liver damage in aflatoxin B1 poisoning

Abstract

Aflatoxin B1 is a hepatotoxic and hepatocarcinogenic compound. It is one of the common mycotoxins that causes various pathological conditions in humans and animals such as acute and chronic liver damage, neurological disorders, neoplasms and reproductive disorders.The present work was aimed to study the preventive effect of a dietary supplement based on milk thistle flour, succinic acid, bentonite, probiotic strain B. subtilis, vitamins A and E in experimental aflatoxicosis of laboratory animals. The experiments were carried out on 24 white rats, divided according to the principle of analogues into 4 groups. The first group - biological control, the second group - toxic control, the third and fourth groups were given medications against mycotoxin. White rats were injected with aflatoxin B1 with food at a dose of 500 µg/kg for 10 days. The treatments effectiveness was assessed by clinical signs of experimental animals and hemato-biochemical analysis. The level of exposure to aflatoxin B1 was evaluated by the state of the liver. The use of a biologically active additive significantly reduced the toxic effect of aflatoxin B1 on the animal organism, prevented a decrease in the amount of hemoglobin, leukocytes and erythrocytes, an increase in platelets, the activity of aminotransferases (aspartate aminotransferase and alanine aminotransferase), alkaline phosphatase and malonic daldehyde. There were no pathoana tomic changes in the internal organs of animals receiving a biologically active supplement.The results of the experiment indicate the high therapeutic efficacy of the biologically active additive used in aflatoxicosis, due to the multicomponent composition.