Pharmacological characterization and distribution of muscarinic receptors in human placental syncytiotrophoblast brush-border and basal plasma membranes

Abstract

Based on the existence of choline acetyltransferase and acetylcholine in human placenta, we have investigated the presence of muscarinic acetylcholine receptors in brush-border and basal plasma membranes from human term placenta. Radioligand binding assay, using [ 3H] N-methyl-scopolamine as tracer, showed the existence of acetylcholine muscarinic receptors in brush-border ( K d 0.28±0.04 nM; B max 9.4±1.6 fmol/mg protein) and basal plasma membranes ( K d 0.24±0.05 nM; B max 34.3±6.3 fmol/mg protein). In order to perform a pharmacological characterization of these receptors, competition binding experiments were carried out using the muscarinic receptor antagonists pirenzepine, (11(2-diethyl-amino)methyl)-1-piperidinylacetyl-5-11-dihydro-6 H-pyrido(14)benzodiazepine (AF-DX 116), himbacine, 4-diphenylacetoxy- N-methylpiperidine methiodide (4-DAMP), dicyclomine and hexahydro-sila-difenidol (HHSD). The results obtained showed that the muscarinic receptors in brush-border and basal plasma membranes belong to different subtypes. In brush-border membranes, the receptor found match in terms of affinity for the antagonists with the muscarinic M 1 receptor subtype ( K i pirenzepine, 13.6±8.2 nM; K i AF-DX 116, 1680±271 nM; K i himbacine, 212±6.5 nM; K i 4-DAMP, 1.5±0.4 nM; K i dicyclomine, 5.1±0.8 nM; K i HHSD, 34.3±7.3 nM), whereas the receptor in basal plasma membrane seems to be of the muscarinic M 2 receptor subtype ( K i pirenzepine, 202±48 nM; K i AF-DX 116, 124±60 nM; K i himbacine, 20.6±4.8 nM; K i 4-DAMP, 4.5±1.2 nM; K i dicyclomine, 54.6±22 nM; K i HHSD, 89.2±15.8 nM). The results obtained show the existence of muscarinic acetylcholine receptors in brush-border and basal plasma membranes from human term placenta with a different distribution pattern in terms of number of receptors and distribution of different subtypes. The functional significance of these findings is as yet unknown, but these receptors probably mediate different functions as they belong to different subtypes and are coupled to different second messengers.