Pharmacological and Biochemical Evaluation of Anti-arthritic Activity of Punica Granatum Extracts in FCA Induced Arthritis in Wistar Rats

Abstract

The present studies were executed on Freund's complete adjuvant (0.1 ml) induced arthritic Wistar rats to explore the folkloric use of the seeds. This study describes the effect of Punica granatum Linn's Ethanolic (PGSE) and Chloroform (PGSC) extract within the FCA-induced arthritis rat paw oedema, vagaries in behaviour, haematological and alterations in biochemical parameters in the developed and progression of arthritis phases. There was a significant rise in the paw swelling (volume) of rats and reduction in (BW) body weight, with FCA-induced arthritic rats. In contrast, PGSE and PGSC with the dose of 200mg/kg and 400mg/kg and Diclofenac (20mg/kg) treated group showed a substantial decrease in paw volume and the normal improvement in body weight to the positive control group. The altered level of haematological parameters, including, Hb, RBC, WBC, and ESR, in arthritic rats, have been substantially regained to normal by PGSE and PGSC treatment at the dosage of 200 mg/kg/p.o and 400 mg/kg/p.o in both the developed and progression of arthritis phases. In this study, Anti-inflammatory action of the PGSE and PGSC with carrageenan-induced paw oedema has also been investigated. Thus, percentage (%) inhibition of PGSE and PGSC were found to be 91.8 % and 86.71, respectively, concerning Diclofenac sodium (93.14%), this gave the evidence of dose-dependent action potential of Punica granatum as anti-inflammatory activity.