Pharmacokinetics of paeoniflorin in Fuke Tiaojing tablets in rat plasma by HPLC

Abstract

Objective To study the pharmacokinetics of paeoniflorin in Fuke Tiaojing tablets in rat plasma by HPLC. Methods Using the paeoniflorin standard and control product of gardenia to find the conditions of HPLC for the detection of the standard curve, and detection of specificity, the minimum detection limit, recovery rate, precision and stability. Twenty rats were divided into two groups: 0.2 g/kg group and 0.4 g/kg group, with 10 rats in each group. Plasma concentration(0.2, 0.5, 1.0, 1.5, 2.0, 4.0, 6.0, 8.0, 12.0, 15.0, 18.0 h after administration) was determined by HPLC and the pharmacokinetics were measured. Results ①The paeoniflorin standard and control product of gardenia had the good peak and specificity by HPLC; peak area of paeoniflorin (Y) on the concentration of sample (X) regression equation: Y=135.598X+ 3.520 (R=0.9999), the lowest detection limit was 0.1 mg/L, and had good measured recovery rate, precision and stability. ②The main pharmacokinetic of 0.2 g/kg group and 0.4 g/kg group were as follow: Cmax were (15.80±1.30) and (21.60±1.70)mg/L, Tmax were (3.52±0.51) and (3.61±0.64)h, AUC0t were (4 312±155) and (5 314±142)(mg·h)/L, AUC0∞ were (5 873±209) and (6 719±174)(mg·h)/L. Conclusions The method of HPLC is simple, sensitive, accurate and suitable for content determination and pharmacokinetic of paeoniflorin in Fuke Tiaojing tablets. Key words: Paeoniflorin; Fuke Tiaojing tablets; HPLC; Pharmacokinetic