Pharmacokinetics of florfenicol and its metabolite florfenicol amine in crucian carp (Carassius auratus) at three temperatures after one single intramuscular injection

Abstract

The pharmacokinetic profiles of florfenicol (FF) or florfenicol amine (FFA) in crucian carp were compared at different water temperatures after single intramuscular administration of FF at 10mg/kg bodyweight. The concentrations of FF and FFA were determined by a high-performance liquid chromatography method, and then, the concentration versus time data were subjected to compartmental analysis using a one-compartment open model. At the water temperatures of 10, 20, and 25°C, the peak concentrations (Cmax s) of FF were 2.28, 2.29, and 2.34μg/ml, respectively, while those of FFA were 0.42, 0.71, and 0.82μg/ml, respectively. And the absorption half-life (t1/2ka ) of FF was 0.21, 0.19, and 0.21hr, while the elimination half-life (t1/2kel ) was 31.66, 24.77, and 21.48hr, respectively. For FFA, the formation half-life (t1/2kf ) was 3.85, 8.97, and 12.43hr, while the t1/2kel was 58.34, 30.27, and 21.22hr, respectively. The results presented here demonstrated that the water temperature had effects on the elimination of both FF and FFA and the formation of FFA. Based on the T>MIC values calculated here, to treat the infections of bacterial with MIC value≤0.5μg/ml, FF intramuscularly given at 10mg/kg bodyweight with a 72-hr interval is sufficient at the water temperature of 10°C, while the intervals of 60 and 48hr were needed at 20 and 25°C, respectively. But to treat bacterial with higher MIC values, more FF or FF at 10mg/kg BW but with shorter intervals should be intramuscularly given to the infected fish.