Pharmacokinetics of Caffeic Acid, Ferulic Acid, Formononetin, Cryptotanshinone, and Tanshinone IIA after Oral Administration of Naoxintong Capsule in Rat by HPLC-MS/MS

Jin Li,Mingrui An,Yun Bai,Jun Cao,Yan-Xu Chang,Yang Bai,Ruichao Zhu,Wei Liu,Zhijing Tan

doi:10.1155/2017/9057238

Abstract

Naoxintong capsule (NXTC) was a famous patent medicine of Traditional Chinese Medicine (TCM) to treat cerebrovascular diseases in China. An LC-MS/MS method was developed for simultaneous determination of 11 major ingredients (paeoniflorin, ecdysterone, amygdalin, mulberroside A, caffeic acid, ferulic acid, salvianolic acid B, astragaloside IV, formononetin, cryptotanshinone, and tanshinone IIA) in NXTC in rat plasma. All analytes were separated on an Eclipse plus C18 column using a gradient mobile phase system of acetonitrile-0.1% formic acid aqueous solution. The lower limits of quantification of 11 ingredients were between 0.075 and 10 ng mL−1. The precision was less than 15% and the accuracies were between 85% and 115%. The results showed that caffeic acid, ferulic acid, formononetin, cryptotanshinone, and tanshinone IIA could be detected after oral administration of NXTC. The validated method was successfully applied to pharmacokinetic study of the caffeic acid, ferulic acid, formononetin, cryptotanshinone, and tanshinone IIA in rats after oral administration of NXTC at single and triple dose.

Highlights

Traditional Chinese Medicine (TCM) prescriptions have played an important role in prevention and treatment of diseases for thousand years in some Asia countries such as China, Japan, and Korea
Geniposide, chlorogenic acid, puerarin, and sennoside B were selected as internal standards to determine glycoside, phenolic acids, flavonoids, and tanshinone, respectively
The results showed that the oral dose of paeoniflorin, ecdysterone, amygdalin, mulberroside A, caffeic acid, ferulic acid, salvianolic acid B, astragaloside IV, formononetin, cryptotanshinone, and tanshinone IIA was 831.7 μg kg−1, 22.9 μg kg−1, 1101.7 μg kg−1, 39.5 μg kg−1, 7.1 μg kg−1, Table 5: 24 h storage at room temperature stability, three freeze-thaw cycles stability and long-term stability of eleven compounds (n = 6)

Summary

Introduction

Traditional Chinese Medicine (TCM) prescriptions have played an important role in prevention and treatment of diseases for thousand years in some Asia countries such as China, Japan, and Korea. Several TCMs were usually combined together as a prescription which is the main form of TCMs to improve the therapeutic efficacy and reduce the possible adverse effect through synergistic action in clinic. Most of the TCM prescriptions were applied to treating some diseases from the ancient times and their therapeutic effects have been demonstrated and proved by broad clinical practice. Buyanghuanwutang, one of the famous TCM prescriptions from the ancient medical book Yi Lin Gai Cuo written by Qingren Wang in Qing Dynasty, mainly records the theory “promoting blood circulation and removing blood stasis.”. Naoxintong capsule (NXTC) is a patent medicine designed on the base of Buyanghuanwutang. It has been widely used in China to treat cerebrovascular disease caused by deficiency of Qi and blood stasis such as hemiplegia and stroke. NXTC contains 11 plant medicines (Astragali Radix (Huangqi), Paeoniae Radix Rubra (Chishao), Salviae miltiorrhizae Radix et Rhizoma (Danshen), Persicae Semen (Taoren), Angelicae Sinensis Radix (Danggui), Achyranthis bidentatae Radix (Niuxi), Chuanxiong Rhizoma (Chuanxiong), Spatholobi Stem (Jixueteng), Cinnamomi Ramulus (Guizhi), Carthami Flos (Honghua) and Mori Ramulus (Sangzhi)), two resins

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Conclusion