Abstract

Asarum is a traditional medicine and has been widely used as remedies for inflammatory diseases, toothache, headache, local anesthesia, and aphthous stomatitis in China, Japan, and Korea. Our previous research found that safrole and methyl eugenol were vital compounds that contribute to distinguish the different species and raw Asarum and its processed products apart. The pharmacokinetics of safrole and methyl eugenol after oral administration of Asarum extract has not been reported yet. In this study, a rapid and simple gas chromatography–mass spectroscopy (GC–MS) method that has a complete run time of only 4.5 min was developed and validated for the simultaneous determination and pharmacokinetic study of safrole and methyl eugenol in rat plasma after administration of Asarum extracts. The chromatographic separation was realized on a DB-17 column (30 m × 0.25 mm × 0.25 μm). And detection was carried out under selected ion monitoring (SIM) mode. Plasma samples were pretreated by n-hexane. The pharmacokinetic parameters provided by this study will be beneficial for further developments and clinical applications of Asarum.

Highlights

  • Asarum, with the Chinese name Xixin, is a perennial plant that is widely distributed in China, Japan, and Korea

  • The specificity was measured by comparing the chromatograms of blank plasma, blank plasma spiked with analytes and IS, and plasma samples obtained after oral administration of Asarum essential oil extracts

  • Results showed that liquid–liquid extraction (LLE) with n-hexane and ethyl acetate both could obtain reproducible extraction and good recovery for the analytes, while the protein precipitation extraction with methanol and acetonitrile was found to contain endogenous interference

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Summary

Introduction

With the Chinese name Xixin, is a perennial plant that is widely distributed in China, Japan, and Korea. Asarum is a traditional medicine and has been widely used as remedies for inflammatory diseases, toothache, headache, local anesthesia, and aphthous stomatitis in many countries [2] Phytochemical studies on this herb showed that the components of Asarum included volatile oil, flavonoids, aristolochic acids, and furofurans-type lignan [3, 4]. BioMed Research International indicated that the safrole and methyl eugenol were identified as key compounds that can be beneficial to differentiate the different species apart Apart from that, both of these two volatile oils were notable index constituents to differentiate raw Asarum and its processed products [14]. This method has a complete run time of only 4.5 min, and it was first used for the pharmacokinetic analysis of safrole and methyl eugenol in rat plasma simultaneously after administration of Asarum essential oil extracts

Material and Methods
Method Validation
Results and Discussion
Conclusion
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