Pharmacokinetic Comparison of Seven Major Bio-Active Components in Normal and Blood Stasis Rats after Oral Administration of Herb Pair Danggui-Honghua by UPLC-TQ/MS.

Yi Jin,Shao-Ping Li,Zhi-Shu Tang,Jin Wang,Xing Chang,Ya-Jun Shi,Yu-Ping Tang,An Kang,Yan-Yan Chen,Jin-Ao Duan,Han-Qing Pang,Gui-Sheng Zhou,Xu-Qin Shi,Jin Sun,Zhen-Hua Zhu,Er-Xin Shang

doi:10.3390/molecules22101746

Abstract

The compatibility between Danggui (Angelicae Sinensis Radix) and Honghua (Carthami Flos) is a known herb pair, which could activate blood circulation and dissipate blood stasis effects. In this paper, we quantified seven main bio-active components (hydroxysafflor yellow A, caffeic acid, p-coumaric acid, kaempferol-3-O-rutinoside, ferulic acid, 3-n-butylphthalide, and ligustilide) in plasma samples in vivo by UPLC-TQ/MS method and investigatedwhether the pharmacokinetic (PK) behaviors of the seven components could be altered in blood stasis rats after oral administration of the Gui-Hong extracts. It was found that the Cmax and AUC0-t of these components in blood stasis rats had increasing tendency compared with normal rats. Most components in model and normal rats had significant difference in some pharmacokinetic parameters, which indicated that the metabolism enzymes and transporters involved in the metabolism and disposition of these bio-active componentsmay bealtered in blood stasis rats. This study was the first report about the pharmacokinetic investigation between normal and blood stasis rats after oral administrationof Gui-Hong extracts, and these results are important and valuable for better clinical applications of Gui-Hong herb pair and relatedTCM formulae.

Highlights

IntroductionThe compatibility between Danggui and Honghua could be found in Danggui-Honghua Decoction of Shanghandabai
Danggui (Angelicae Sinensis Radix) is the dried radix of Angelica sinensis (Oliv.) Diels (Umbelliferae) and Honghua (CarthamiFlos) is the dried flower of Carthamus tinctorius L. (Compositae).The compatibility between Danggui and Honghua could be found in Danggui-Honghua Decoction of Shanghandabai
The haemorheological indices of the rats in these two groups are shown in Table 1, including whole blood viscosity (WBV), plasma viscosity (PV), erythrocyte sedimentation rate (ESR), andhematocrit (HCT)

Summary

Introduction

The compatibility between Danggui and Honghua could be found in Danggui-Honghua Decoction of Shanghandabai. It is an ancient and classic formula comprised of Danggui and Honghua, and it is a Molecules 2017, 22, 1746; doi:10.3390/molecules22101746 www.mdpi.com/journal/molecules. Pharmacological studies and chemical investigation have found that the aromatic acids, phthalides and quinochalcone C-glycosides were the main constitutes responsible for the herb pair’bio-activities [2,3]. Pharmacokinetic studieson these components havebeen partly demonstrated [4,5,6]. The pharmacokinetic process of herbs will be affectedby the co-usedherbs when used as an herb pair or herb formulae [7]

Methods

Results

Conclusion