Abstract
In the present study, post inflammation irritable bowel syndrome (PI-IBS) rats were firstly established by intracolonic instillation of acetic acid with restraint stress. Then the pharmacokinetics of berberine in the rat plasma were compared after oral administration of berberine hydrochloride (25 mg/kg) to normal rats and PI-IBS rats. Quantification of berberine in the rat plasma was achieved by using a sensitive and rapid UPLC-MS/MS method. Plasma samples were collected at 15 different points in time and the pharmacokinetic parameters were analyzed by WinNonlin software. Compared with the normal group, area under the plasma concentration vs. time curve from zero to last sampling time (AUC0–t) and total body clearance (CL/F) in the model group significantly increased or decreased, (2039.49 ± 492.24 vs. 2763.43 ± 203.14; 4999.34 ± 1198.79 vs. 3270.57 ± 58.32) respectively. The results indicated that the pharmacokinetic process of berberine could be altered in PI-IBS pathological conditions.
Highlights
Berberine is a botanical alkaloid which is present in many plants and has been used for many years due to its inexpensiveness and low incidence of adverse effects [1]
The quantity of the mast cells in the model group increased remarkably. These results indicated that intracolonic instillation of acetic acid with restraint stress could cause anomaly of mast cells
post inflammation irritable bowel syndrome (PI-IBS) rat models were established by intracolonic instillation of acetic acid to induce acute inflammation of the colon; after the inflammation resolved, wrap restraint stress was given to the rats
Summary
Berberine is a botanical alkaloid which is present in many plants and has been used for many years due to its inexpensiveness and low incidence of adverse effects [1]. It has been traditionally used as an antimicrobial agent initially, and achieved favorable therapeutic effects in treating diarrhea. More and more research shows that the pharmacokinetic parameter of drugs can be affected by the disease states [15,16,17,18,19,20,21]. The pharmacokinetics of berberine in rat plasma were compared after oral administration of berberine hydrochloride in normal and PI-IBS rats by UPLC-MS/MS methods
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