Pharmacokinetic Analysis of Four Bioactive Iridoid and Secoiridoid Glycoside Components of Radix Gentianae Macrophyllae and Their Synergistic Excretion by HPLC-DAD Combined with Second-Order Calibration

Tian-Ming Yang,Hai-Long Wu,Qiao-Bo Yin,He-Dong Li,Li-Ping Wang,Hai-Yan Fu,He-Bin Tang,Yang-Xi Liu,Wei Lan,Han-Bo Su

doi:10.1007/s13659-017-0145-7

Abstract

An HPLC-DAD method combined with second-order calibration based on the alternating trilinear decomposition (ATLD) algorithm with the aid of region selection was developed to simultaneously and quantitatively characterize the synergistic relationships and cumulative excretion of the four bioactive ingredients of Radix Gentianae Macrophyllae in vivo. Although the analytes spectra substantially overlapped with that of the biological matrix, the overlapping profiles between analytes and co-eluting interferences can be successfully separated and accurately quantified by the ATLD method on the basis of the strength of region selection. The proposed approach not only determined the content change but also revealed the synergistic relationships and the cumulative excretion in vivo of the four ingredients in urine and feces samples collected at different excretion time intervals. In addition, several statistical parameters were employed to evaluate the accuracy and precision of the method. Quantitative results were confirmed by HPLC-mass spectrometry. Satisfactory results indicated that the proposed approach can be utilized to investigate the pharmacokinetics of Radix Gentianae Macrophyllae excretion in vivo.Graphical

Highlights

An HPLC-diode array detector (DAD) method combined with second-order calibration based on the alternating trilinear decomposition (ATLD) algorithm with the aid of region selection was developed to simultaneously and quantitatively characterize the synergistic relationships and cumulative excretion of the four bioactive ingredients of Radix Gentianae Macrophyllae in vivo
Radix Gentianae Macrophyllae belongs to the Gentiana genus of Gentianaceae and is widely used as a remedy in traditional Chinese medicine (TCM) for more than 2000 years [1]
One can resort to the second-order calibration method based on ATLD algorithm, which allows the relative chromatographic, spectral, and concentration profiles of analytes to be extracted even in the presence of uncalibrated interferences

Summary

Introduction

Radix Gentianae Macrophyllae belongs to the Gentiana genus of Gentianaceae and is widely used as a remedy in traditional Chinese medicine (TCM) for more than 2000 years [1]. The dominant bioactive constituents in Radix Gentianae Macrophyllae are iridoid and secoiridoid glycosides, including gentiopicroside (GPS), loganic acid (LOG), swertiamarin (SWM), and sweroside (SWS), which exhibit analgesic, anti-inflammatory, antipyretic, antirheumatic, diuretic, febrifuge, and hypoglycemic pharmacological effects for treating hypotension, rheumatism, pains, fever, and allergic inflammations [2,3,4,5,6]. Unlike those of chemical drugs, the therapeutic effects of TCM are based on the synergistic effect of their bioactive compounds [7].

Methods

Results

Conclusion