Abstract
Spinach (Spinacia oleracea) is an edible flowering plant in the family Amaranthaceae native to central and western Asia. Its leaves are eaten as a vegetable. Spinach has a high nutritional value because it is a rich source of vitamin A, vitamin C, vitamin K, magnesium, manganese, iron , riboflavin vitamin B6, vitamin E, calcium, potassium, and dietary fiber. Pharmacognostic standardization, physicochemical evaluation of the Leaves of Spinacia oleracea was carried out to determine its macro-and microscopical characters and also total ash, insoluble ash, alcohol- and water-soluble extractive values were determined for phytochemical evaluations. Preliminary phytochemical screening was also done to detect different phytoconstituents. The Proximate analysis of powder was also carried out in which extractive value, ash value, foreign matter, loss on drying were determined and also successive solvent extraction was carried out using soxhlet extractor in which ethanol was used. Aqueous extract was also carried out by maceration method. Preliminary phytochemical screening of various successive extracts of leaves was done qualitatively which revealed the presence of phytosterol, saponins, tannins, flavonoids, carbohydrates, tannins and amino acids.
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