Pharmacognostical, Phytochemical and In Vitro antiglycation studies on the leaf extracts of Hibiscus micranthus Linn.

Abstract

The aim of the present study was to carry out powder analysis, fluorescence analysis, microscopic study, physico chemical analysis, total flavonoid and phenolic content and in vitro antiglycation studies for the leaf aqueous and alcoholic extracts of Hibiscus micranthus Linn. by serum albumin assay. The results of powder analysis indicated that the powder with ferric chloride solution showed mustard green colour, under UV powder with ferric chloride showed brown colour. The total ash value 75%w/w, acid insoluble ash 25%w/w, water soluble ash 35%w/w and sulphated ash 15%w/w respectively. The water soluble extractive value 2.2%w/w, alcohol soluble extractive 19.3%w/w and the result of loss on drying were found to be 9.33%w/w. The microscopic study observations were presence of stellate 3–4 armed trichomes, anisocytic stomata, mucilaginous parenchyma, lignified libiform xylem vessels and Ca.oxalate druse crystals. The leaf constant values include stomatal index of upper and lower epidermis to be 4.66, 4.15, palisade ratio 9, vein islet number 12, veinlet termination number to be 9. The length and width of xylem vessels to be 15.75μ, 117.62 μ. The length and width of trichomes was found to be 30.6 μ and 2.87 μ. The results of preliminary phytochemical screening showed the presence of alkaloids, tannins, phenolics, flavonoids, steroids, cardenolides, coumarin glycosides, reducing sugars, acidic compounds, inulin, waxes and reserpine. The total flavonoid content was more in methanolic extract 104±0.36 mg/g equivalents of rutin. The phenolic content was also more in methanolic extract 52.76±0.67 mg/g equivalents of rutin. The methanolic extract exhibited significant in vitro antiglycation activity at 1000 μg/ml 95.6%±0.0025** equivalent to standard drug rutin. Therefore the individual phytoconstituents from the extracrs can be further explored and studied for the activity.