Pharmacognostic Study of Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) and its Clay Mask Against Acne-Causing Bacteria

Abstract

<a name="_Hlk53342230">Previous studies have shown that the ethanolic extract of Bawang Dayak (Eleutherine bulbosa (Mill.) Urb.) can inhibit acne-causing bacteria. Therefore, this study aims to conduct a pharmacognostic evaluation of Bawang Dayak and make a clay mask for acne vulgaris. Bawang Dayak bulbs were extracted using a percolator with 96% ethanol. The qualitative test is carried out using several methods, including the identification of organoleptic, macroscopic, microscopic, and chemical compounds with thin-layer chromatography. Furthermore, the physical and microbiology test was conducted on the clay mask using the disc diffusion technique against acne-causing bacteria. The result showed the physico-chemical parameters of bulbs as 8.67% loss on drying, 0.21% foreign organic matter, 13.31% water-soluble, 10.52% alcohol soluble, 0.53% total ash, 0.85% acid insoluble ash, and 1.67% water-soluble ash. The qualitative test of the phytochemical compound showed the presence of alkaloids, flavonoids, saponins, tannins, and catechols. The best eluent TLC for the ethanolic extract was n-hexane: ethyl acetate (8:2) or ethyl acetate: ethanol: water (8:2:1). Furthermore, the clay mask has moderate activity against S. aureus and S. epidermidis and weak activity against P.acnes. Conclusively, pharmacognostic studies on Bawang Dayak simplicia bulbs met the set parameters, and the clay mask of Bawang Dayak inhibits acne-causing bacteria.<o:p></o:p>