Pharmacognostic study and anti–inflammatory activity of Callistemon lanceolatus leaf

Abstract

Objective To study detail pharmacognosy and anti–inflammatory activity of Callistemon lanceolatus ( C. lanceolatus) leaf. Methods Leaf sample was studied by organoleptic, macroscopical, microscopical, phytochemical and other WHO recommended methods for standardizations. The methanolic leaf extract of the plant was also screened for anti–inflammatory activity on carrageenan-induced paw edema in rat at doses of 200 and 400 mg/kg, orally. The detail pharmacognostic study of the C. lanceolatus leaf was carried out to lay down the standards which could be useful in future experimental studies. Results C. lanceolatus methanolic leaf extract showed significant ( P < 0.05) anti–inflammatory activity at doses of 200 mg/kg and 400 mg/kg. This significant anti–inflammatory of C. lanceolatus methanolic leaf extract at the dose of 400 mg/kg was comparable with diclofenac sodium. Conclusions The pharmacognostic profile of the C. lanceolatus leaf is helpful in standardization for quality, purity and sample identification. The methanolic extract at a dose of 400 mg/kg shows a significant activity in comparison with the standard drug diclofenac sodium (50 mg/kg).