Abstract
A new simple and effective routine analytical method for quantification of curcuminoids in Curcuma xanthorrhiza was developed by high-performance liquid chromatography. This method based on chromatographic fingerprint combined with a quantitative analysis of multi-components by single marker (QAMS). Curcumin was selected as an internal marker for the determination of two other similar compounds, i.e. bisdemethoxycurcumin and demethoxycurcumin, by using the relative coefficient factor (RCF). Excellent linearity was obtained for each component (r 2 > 0.9998), and the recovery of extraction methods were within 100.23-103.95%. The precision of the method was good at inter-day and intra-day analysis (RSD < 4.0%). The stability of RCFs was good under various chromatographic conditions with RSD < 1%, and the ratio of retention time was used to locate each compound. The quantification of curcuminoids between QAMS and external standard method (ESM) proved the consistency and similarity of the two method (RSD < 2%). This study demonstrated that QAMS could be used as a routine method for quality control of curcuminoids in C. xanthorrhiza . This method successfully proved accurate, stable, more effective and simple than external standard method.
Highlights
The problem of the global spread of acute intestinal infections (AII) is currently relevant, as they occupy one of the leading places among infectious diseases, yielding only the frequency of influenza and acute respiratory illness
Simulation results showed normally distributed values of critical rotavirus prevalence with expected one of 0.815 (Figure 5). This result means that zinc supplementation is justified in case if rotavirus prevalence among patients with acute diarrhea will be more than 81.5%
Comparing this expected value with the real rotavirus prevalence among patients in the clinical database (p = 0.527), we found that zinc inclusion into acute diarrhea treatment would be rational from pharmacoeconomic point of view for patients aged less than five years in Indonesia in case of higher rotavirus prevalense rate (>81.5%)
Summary
The problem of the global spread of acute intestinal infections (AII) is currently relevant, as they occupy one of the leading places among infectious diseases, yielding only the frequency of influenza and acute respiratory illness Among AII, rotavirus infection (RVI) remains the leading cause of morbidity and mortality in children under five years of age, despite a reduction in the number of cases of hospitalization associated with diarrhea and death (Lanata et al, 2013). Rotavirus causes severe diarrhea and vomiting, leading to significant loss of bodily fluids and resulting in dehydration. The greatest advancement in the treatment of diarrhea in children has been achieved through the introduction of World Health Organization (WHO) recommendations, oral rehydration protocols. These treatment regimens are aimed only at dehydration correction and do not reduce the frequency, volume of emptying or duration of diarrhea (Sato and Zouain, 2010). According to a number of randomized clinical trials, it has been established that the administration of zinc during diarrhea and several days after the onset of acute gastroenteritis reduces the duration and severity of diarrhea, and prevents the emergence of further cases of gastroenteritis for at least several months (Walker et al, 2010; Roy et al, 2018)
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