Pharmaco bacteriological investigation of erythromycin, cephalexin, cephradine, amoxycillin and ciprofloxacin against different strains of klebsiella pneumonaie, escherichia coli, pseudomonas aeruginosa, staphylococcus aureus and salmonella species

Abstract

Different groups of antibiotics (quinolones, macrolides, penicillin and cephalosporins) were chosen and used in this study. These antibiotics included ciprofloxacin, erythromycin, amoxycillin, cephalexin and cephradine respectively. Twenty six bacterial species were collected from urine, milk, rectal swabs, liver, wounds, intestine and also from isolated culture which were supplied by the department of microbiology, faculty of veterinary medicine, university of Khartoum, Sudan. Identification was done by using identification kits namely Quick GN “Nissui” and also by using biochemical tests as confirmatory tests. The bacterial species were found to be: Klebsiella pneumonaie, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and six strains of Salmonella species. Sensitivity tests were performed for all these organisms against various antibiotics with different concentrations using standard disk diffusion method. Ciprofloxacin was found to be the most effective drug against all the organisms tested even at a very low concentration (0.781 µg /ml), all the Gram-negative bacteria were found to be resistant to erythromycin. Pseudomonas aeruginosa strains were found to be highly sensitive to ciprofloxacin and resistant to the most of the other groups of antibiotics. Quinolon group (Ciprofloxacin, ofloxacin and pefloxacin) was found to be the most effective group against Salmonella typhi Keywords: Erythromycin, Cephalexin, Cephradine, Amoxycillin, Ciprofloxacin, E. coli, Klebseilla, Salmonella spp.