Abstract
Gas chromatography is an analytical tool for the separation of compounds in complex mixtures based on the polarity of compounds. Separation is achieved only for compounds that are volatile or that can be made volatile on derivatization of the compound using derivatizing agents. This is one of the widely accepted tools for the separation of compounds because of its simplicity, sensitivity, and effectiveness. The principle of separation of compounds depends on the partitioning behaviour difference between mobile and stationary phase, the sample is carried by a moving gas stream through a tube packed with a finely divided solid or may be coated with a film of a liquid. Different types of columns having a various composition of stationary phase are been used for the separation of different classes of compounds mixture or sample in a suitable solvent is introduced through the injector maintained at higher temperature which is capable of volatilizing the compound into the column.
Highlights
Sample gets adsorbed in the stationary phase of the column which is separated by the carrier gas flowing through the column based on the polarity
Different types of columns having a various composition of stationary phase are been used for the separation of different classes of compounds mixture or sample in a suitable solvent is introduced through the injector maintained at higher temperature which is capable of volatilizing the compound into the column
Gas chromatography coupled with mass spectrometry has been extensively used in the field of metabolomics study even though this is not been associated with study involving volatile organic compounds
Summary
Sample gets adsorbed in the stationary phase of the column which is separated by the carrier gas flowing through the column based on the polarity. Different compounds have different RT based on the polarity [1] The response of such Gas Chromatography detectors is proportional to the concentration of the analyte in the introduced sample. Various applications of GC in pharmaceutical fields are: 1) Residual solvent analysis; 2) Analysis of various functional groups; 3) Percentage of purity of pharmaceutical compounds; 4) For the analysis of drugs of abuse; 5) In pharmaceutical R & D’s to determine the identity of natural products which contains complex mixture of similar compounds; 6) In the metabolomics studies. This assignment discusses the various applications of GC in pharmaceutical fields and the advantages and disadvantages
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