Abstract
In order to confirm the hybrid origin of Phalaenopsis × intermedia Lindl., the internal transcribed spacers (ITSs) of ribosomal DNAs (rDNAs) and three fragments of chloroplast DNAs (cpDNAs) were studied. Nineteen clones of ITS sequences from three individuals of P. × intermedia Lindl. were aligned with 9 species of sections Phalaenopsis and Stauroglottis that are candidate parents. The phylogenetic tree derived from ITS data was constructed by the Neighbor-joining (NJ) method. Two major groups were shown for 19 clones of ITS sequences of P. × intermedia based on phylogenetic tree. The average genetic distance between aforementioned two groups and candidate parents was calculated based on Kimura 2-parameter method. One group has the lowest genetic distance referring to ITS repeat sequences of P. aphrodite Rchb.f., and P. equestris (Schauer) Rchb.f. is that of another. The results indicated that both P. aphrodite Rchb.f. and P. equestris (Schauer) Rchb.f. are parents of P. × intermedia based on ITS data. In addition, analysis of three fragments of cpDNAs, namely trnL intron, trnL-trnF intergenic spacer (IGS), and atpB-rbcL IGS, showed that P. × intermedia Lindl. has a lowest genetic distance with P. aphrodite Rchb.f. From both ITSs and cpDNAs data, as well as referring the property of maternal inheritance of cpDNAs, it suggested that P. aphrodite Rchb.f. is the maternal parent and P. equestris (Schauer) Rchb.f. is the paternal parent of P. × intermedia Lindl. Therefore, from these molecular evidence we support P. × intermedia Lindl. is a natural hybrid derived from P. aphrodite Rchb.f. and P. equestris (Schauer) Rchb.f.
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