Abstract
Defibrination of fresh, peripheral, venous blood from three human adult volunteers resulted in the removal of from 115 to 1/2 of leukocytes, as compared with EDTA-anticoagulated aliquots from identical blood samples. However, differential white blood cell proportions were altered only marginally. Bacterial inocula (approximately 1.5 x 104 bacteria/ml at 0 time) of selected assay strains of Serratia marcescens were killed by 65 vol% of fresh, defibrinated human blood to the extent of 97% within 2 hours after exposure, regardless of serum susceptibility or -resistance of the test strains. The addition of 25 vol% of either undiluted or 1:2 diluted commercially available, intravenously applicable, human IgG immunoglobulin preparations (Gamma-Venin®, Sandoglobulin®) to 65 vol% of fresh, defibrinated blood from all three human volunteers failed to enhance combined phagocytic and serum bactericidal activity against all assay strains of S. marcescens examined, despite documented O-agglutinin activity of the IgG immunoglobulin preparations against the majority of the test strains. It was concluded tentatively that ‘natural’ human specific anti-O IgG antibodies failed to enhance phagocytosis in this in vitro system.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
