Abstract
In this paper, we describe the application of electroporation to deliver phage DNA into bacterial cells in order to recover it as phage particles. The methodology represents a quicker and cheaper alternative to the use of packaging extracts to rescue phage clones stored as naked DNAs. Furthermore, our data demonstrate that there were not rearrangements or recombinations between phage DNAs when a mixture of different DNAs was electroporated, suggesting the use of electroporation as a reliable method for construction of gene libraries.
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