Abstract
Phage displayed domain antibody mimics for pyrethroid and its application in immunoassay
Highlights
The presence of small molecule chemical contaminants, such as pesticides, mycotoxins, antibiotics and veterinary drugs in foods have attracted great attentions of society since they pose a serious threat to human health
The ability of the library to bind to anti-phenoxybenzoic acid (PBA) monoclonal antibody (MAb) was detected by polyclonal ELISA
Three different phage clones (A8, B8, C6) which was illustrated strong binding capacity to anti-PBA MAb were obtained in present study
Summary
The presence of small molecule chemical contaminants, such as pesticides, mycotoxins, antibiotics and veterinary drugs in foods have attracted great attentions of society since they pose a serious threat to human health. It is necessary to develop a simple and rapid analytical technique for monitoring the residue of small chemicals in foods and feedstuffs[1,2]. The conventional competing antigens used for the development of a competitive immunoassay are synthesized by a chemical process. When the hapten is toxic, it may lead a threat to environment and human health[6,7]. It is important in developing an environment-friendly method to obtain the competing antigens
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