Phaeocystidibacter marisrubri sp. nov., a member of the family Cryomorphaceae isolated from Red Sea sediment.

Abstract

Strain G18(T), a Gram-stain-negative, aerobic, rod-shaped, motile, non-fermentative, yellow-pigmented bacterium, was isolated from Red Sea sediment. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain G18(T) was most closely related to Phaeocystidibacter luteus PG2S01(T) with 95.3% similarity. Growth of strain G18(T) occurred at 10-42 °C (optimum 28-37 °C), pH 5.0-9.0 (optimum pH 6.0-8.0) and in the presence of 0.5-10% NaCl (optimum 2-5%). The major fatty acids of strain G18(T) were iso-C15 : 0, summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c), iso-C15 : 1 G and iso-C17 : 0 3-OH. The major polar lipids were phosphatidylethanolamine, unidentified aminolipids, phospholipids and other lipids. The predominant quinone was menaquinone 6 (MK-6). The G+C content of the genomic DNA from strain G18(T) was 39.0 mol%. Based on the phylogenetic and phenotypic properties, strain G18(T) represents a novel species of the genus Phaeocystidibacter, for which the name Phaeocystidibacter marisrubri sp. nov. is proposed. The type strain is G18(T) ( = CGMCC 1.14954(T)= JCM 30614(T)).