PH-responsive drug delivery system based on luminescent CaF 2:Ce 3+/Tb 3+-poly(acrylic acid) hybrid microspheres

Abstract

In this study, we design a controlled release system based on CaF 2:Ce 3+/Tb 3+-poly(acrylic acid) (PAA) composite microspheres, which were fabricated by filling the pH-responsive PAA inside CaF 2:Ce 3+/Tb 3+ hollow spheres via photopolymerization route. The CaF 2:Ce 3+/Tb 3+ hollow spheres prepared by hydrothermal route possess mesoporous structure and show strong green fluorescence from Tb 3+ under UV excitation. Doxorubicin hydrochloride (DOX), a widely used anti-cancer drug, was used as a model drug to evaluate the loading and controlled release behaviors of the composite microspheres due to the good biocompatibility of the samples using MTT assay. The composite carriers provide a strongly pH-dependent drug release behavior owing to the intrinsic property of PAA and its interactions with DOX. The endocytosis process of drug-loaded microspheres was observed using confocal laser scanning microscopy (CLSM) and the in vitro cytotoxic effect against SKOV3 ovarian cancer cells of the DOX-loaded carriers was investigated. In addition, the extent of drug release could be monitored by the altering of photoluminescence (PL) intensity of CaF 2:Ce 3+/Tb 3+. Considering the good biocompatibility, high drug loading content and pH-dependent drug release of the materials, these hybrid luminescent microspheres have potential applications in drug controlled release and disease therapy.