Abstract
pH-responsive nanocarrier systems exhibit diverse applications due to their relevance to biological systems where, discrete pH differences are displayed between tissues and cellular compartments. Changes in the physicochemical properties of responsive nanocarriers due to variations in pH, can cause release of their cargos in a targeted and controllable manner offering spatiotemporal control. In this study, bovine serum albumin-poly(2-(diisopropylamino)ethyl methacrylate) (BSA-poly(DPA)) biohybrid nanoparticles were synthesized via controlled radical polymerization and found to be stable in neutral pH (7.4) while, at lower pHs (4–5.5) they were shown to disassemble and release their payload. In vitro tests demonstrated low cytotoxicity and energy-, dose- and time-depended internalization. BSA-poly(DPA) nanocarriers loaded with the metachromatic dye acridine orange, were successfully delivered in HeLa cells where, a pH-triggered release of their cargo into lysosomes was evidenced paving the way for applications related with the treatment of lysosomal diseases.
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