Abstract
In this study, we investigated the behavioral patterns of gelatin (Gel) and Agar in terms of composites and phase separation. As the pH (4.5–7.0) increased, the electrostatic interaction between the gel and the agar weakened, and a decrease in the degree of agglomeration of the microstructure was observed in the confocal laser scanning microscope (CLSM) plots. Specifically, When the ratio of Gel to Agar was 2:1 and the pH was 4.5 (pHopt), a large amount of complex coalescence appeared. The Absorption and fluorescence spectra demonstrated the impact of Agar interaction on the tertiary conformation of the Gel, while circular dichroism (CD) detected changes in its secondary structure. Furthermore, Fourier Transform Infrared Resonance spectroscopy (FTIR) demonstrated that the complexes were produced through electrostatic interactions between the -NH3+ groups of Gel and –COO- groups of Agar. The Gel-Agar coacervation process induced structural modifications, which enhanced the Gel’s thermal stability. The findings of this study are expected to be an essential contribution to the potential application of Gel-Agar coacervates for encapsulating functional ingredients.
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