Abstract
An artificial proton gradient provided sufficient energy to drive reverse electron transport from succinate to NADH:ubiquinone oxidoreductase in chromatophores isolated from Rhodospirillum rubrum. The pH gradient created was able to reduce NAD +. In chromatophores, the optimal rate of NAD + reduction was about 4–45 μmol NADH formed/min · μmol bacteriochlorophyll at ΔpH 3. The presence of oligomycin was an obligate factor in the assay in order to observe the maximal rate of NAD + reduction. The rate of NADH formation was dependent on the size of the induced pH gradient. The total NADH formed had a threshold value for the imposed ΔpH. The effect of different inhibitors and uncouplers was demonstrated. Comparison between ATP, PP i and light with the pH jump driven NAD + reduction rate was studied.
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