Abstract
Spontaneous mammary tumours of the rat with various degrees of malignancy exhibit similar tissue pH distributions. The mean pH (+/- s.d.) of dysplasia is 7.05 +/- 0.20. In benign tumours the mean pH is 6.95 +/- 0.19 and in malignant tumours it is 6.94 +/- 0.19. In contrast, tumours with the same degree of malignancy but different histologies show different pH distributions. Benign tumours with a higher percentage of fibrous tissue exhibit less acidic pH values than those with larger portions of epithelial cells (delta pH = 0.38 pH units). The pH distribution in the benign tumours is independent of the tumour wet weight up to stages of very advanced growth. In the malignant tumours, a trend towards more acidic pH values is observed as the tumour mass enlarges. However, in tissue areas within a malignant tumour with gross, long-established necrosis the pH distribution is shifted towards more alkaline pH values. The pH distributions in spontaneous rat tumours are not significantly different from those obtained in isotransplanted Yoshida sarcomas (6.87 +/- 0.21). In the Yoshida sarcomas, mean pH values do not correlate with tumour size. However, a pH gradient from the rim to the centre of the tumours is found which coincides with the development of small, disseminated necroses in the tumour centre. It is concluded that pathology-related variations of tumour pH may be more important than the mode of tumour origin or the degree of malignancy.
Highlights
PH distributions were measured in isotransplanted Yoshida sarcomas (n = 30)
The Yoshida sarcoma was originally obtained from the German Cancer Research Centre, Heidelberg (FRG), and was serially passaged as ascites tumour iA the abdominal cavity of SD-rats (120-180g)
The pH values obtained during normal acid-base status (Table II) in spontaneous and isotransplanted rat tumours were lower than those measured in the normal tissues at the site of growth
Summary
Spontaneous tumours (n=27), that grew along the milk line of Sprague-Dawley rats were used throughout the experiments. Tumour-bearing animals (both sexes; 225-640 g), obtained from the breeding colonies of Hoechst AG (Frankfurt/M., FRG), from the Department for Animal Experimentation, University of Frankfurt (Frankfurt/M., FRG) and from the Department of Applied Physiology, University of Mainz (Mainz, FRG) were submitted to the study. PH distributions were measured in isotransplanted Yoshida sarcomas (n = 30). The sarcomas grew s.c. in the hind foot dorsum of SD-rats of both sexes (180-560g) after inoculation of ascites cells The Yoshida sarcoma was originally obtained from the German Cancer Research Centre, Heidelberg (FRG), and was serially passaged as ascites tumour iA the abdominal cavity of SD-rats (120-180g). PH values were measured in the subcutis and in skeletal muscle
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